Question

Дайте побыстрее ответ, у меня ограниченное время. Установите последовательность действий Л. Пастера при разработке вакцины против куриной холеры. А. Ослабление возбудителя путём его многочисленной пересадки на питательных средах. Б. Проверка вакцины путём заражения цыплят смертельной дозой возбудителя куриной холеры. В. Выделение микробов (культуры) куриной холеры на питательной среде. Г. Заражение цыплят ослабленными бактериями куриной холеры. Д. Получение ослабленной культуры бактерий куриной холеры.

Answer 1

Ответ:

Б,Д,А,В,Г

Объяснение:

Answer 2

Sequence of Actions by Louis Pasteur in Developing a Vaccine against Chicken Cholera

Louis Pasteur, a renowned French scientist, made significant contributions to the field of microbiology and immunology. He developed several vaccines, including one against chicken cholera. The sequence of actions taken by Pasteur in developing the vaccine can be summarized as follows:

A. Isolation and Cultivation of the Microorganism: Pasteur first isolated the microorganism responsible for chicken cholera and successfully cultivated it in a laboratory setting. This involved growing the bacteria on nutrient media to obtain a pure culture [[1]].

B. Weakening the Pathogen through Serial Passages: To weaken the virulence of the pathogen, Pasteur subjected it to multiple passages on nutrient media. This process, known as attenuation, involved transferring the bacteria from one culture to another over a series of generations. With each passage, the pathogen gradually lost its ability to cause severe disease [[2]].

C. Testing the Vaccine on Animals: After obtaining an attenuated culture of the bacteria, Pasteur tested the vaccine's efficacy by infecting chickens with a lethal dose of the virulent strain of the chicken cholera bacterium. He observed that the vaccinated chickens remained healthy and did not develop the disease. This demonstrated the protective effect of the vaccine [[3]].

D. Mass Production of the Vaccine: Once the vaccine's effectiveness was confirmed, Pasteur scaled up the production process. He prepared large quantities of the attenuated bacteria culture and used it to vaccinate a larger population of chickens. This allowed for the mass production and distribution of the vaccine [[4]].

In summary, the sequence of actions taken by Louis Pasteur in developing a vaccine against chicken cholera involved isolating and cultivating the microorganism, weakening the pathogen through serial passages, testing the vaccine on animals, and mass-producing the vaccine for widespread use.

Please note that the information provided above is based on search results and may not cover all the details of Pasteur's work on the chicken cholera vaccine.